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Test ID NBLD0555 Benzodiazepines Confirmation, Random, Urine


Ordering Guidance


For situations where chain of custody is required, a Chain-of-Custody Kit (T282) is available. For chain-of-custody testing, order BNZX / Benzodiazepines Confirmation, Chain of Custody, Random, Urine.

 

Additional drug panels and specific requests are available. Call 800-533-1710 or 507-266-5700.



Additional Testing Requirements


If urine creatinine is required or adulteration of the sample is suspected, also order ADULT / Adulterants Survey, Random, Urine in addition to this test.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Plastic urine container

Submission Container/Tube: Plastic, 5-mL tube

Specimen Volume: 1 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative

Additional Information:

1. No specimen substitutions.

2. STAT requests are not accepted for this test.

3. Submitting less than 1 mL will compromise our ability to perform all necessary testing.


Secondary ID

608255

Useful For

Detecting drug use involving benzodiazepines such as alprazolam, chlordiazepoxide, clonazepam, diazepam, midazolam, oxazepam, temazepam, clobazam, flunitrazepam, flurazepam, lorazepam, prazepam, triazolam, and zolpidem

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Benzodiazepines Confirmation, U

Specimen Type

Urine

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 7 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Icterus Reject

Reference Values

Negative

 

Cutoff concentrations by liquid chromatography tandem mass spectroscopy:

Alprazolam: 10 ng/mL

Alpha-hydroxyalprazolam: 10 ng/mL

Chlordiazepoxide: 10 ng/mL

Clonazepam: 10 ng/mL

7-Aminoclonazepam: 10 ng/mL

Diazepam: 10 ng/mL

Nordiazepam: 10 ng/mL

Midazolam: 10 ng/mL

Alpha-hydroxy midazolam: 10 ng/mL

Oxazepam: 10 ng/mL

Temazepam: 10 ng/mL

Clobazam: 10 ng/mL

N-Desmethylclobazam: 10 ng/mL

Flunitrazepam: 10 ng/mL

7-Aminoflunitrazepam: 10 ng/mL

Flurazepam: 10 ng/mL

2-Hydroxy ethyl flurazepam: 10 ng/mL

Lorazepam: 10 ng/mL

Prazepam: 10 ng/mL

Triazolam: 10 ng/mL

Alpha-hydroxy triazolam: 10 ng/mL

Zolpidem: 10 ng/mL

Zolpidem phenyl-4-carboxylic acid: 10 ng/mL

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

80347

80339

80368

G0480 (if appropriate)

Day(s) Performed

Monday through Friday

Report Available

2 to 6 days

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen. 

Sanford Laboratories - Sioux Falls/Rapid City Additional Information:

SANFORD LABORATORY INTERFACE BUILD INFORMATION

 Result Code     Result Code Description   
 26006  Alprazolam by LC-MS/MS   
 26007  Alpha-Hydroxyalprazolam by LC-MS/MS   
 26008  Chlordiazepoxide by LC-MS/MS   
 26009   Clonazepam by LC-MS/MS
 26010  7-Aminoclonazepam by LC-MS/MS   
 26011  Diazepam by LC-MS/MS
 26012  Nordiazepam by LC-MS/MS
 26013  Midazolam by LC-MS/MS
 26014  Alpha-Hydroxy Midazolam by LC-MS/MS
 26015  Oxazepam by LC-MS/MS
 26016  Temazepam by LC-MS/MS
 26017  Clobazam by LC-MS/MS
 26018  N-Desmethylclobazam by LC-MS/MS   
 26019  Flunitrazepam by LC-MS/MS
 26020  7-Aminoflunitrazepam by LC-MS/MS
 26021  Flurazepam by LC-MS/MS
 26022  2-Hydroxy Ethyl Flurazepam by LC-MS/MS   
 26023  Lorazepam by LC-MS/MS   
 26024  Prazepam by LC-MS/MS
 26025  Triazolam by LC-MS/MS
 26026  Alpha-Hydroxy Triazolam by LC-MS/MS
 26027  Zolpidem by LC-MS/MS
 26028  Zolpidem Phenyl-4-Carboxylic Acid by LC-MS/MS   
 26029  Benzodiazepines Interpretation