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HelpTest ID LBOR0252 KIT p.Asp816Val Variant Analysis, Quantitative, Varies
Useful For
Diagnosing systemic mastocytosis
Specimen Type
VariesShipping Instructions
Whole blood or bone marrow specimens must arrive within 14 days of collection.
Necessary Information
Specimen type is required to perform testing.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Whole blood
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix.
2. Send specimen in original tube. Do not aliquot.
3. Label specimen as blood.
Specimen Stability Information: Ambient (preferred) 14 days/Refrigerate 14 days
Specimen Type: Bone marrow aspirate
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix.
2. Send specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability Information: Ambient (preferred) 14 days/Refrigerate 14 days
Specimen Type: Extracted DNA
Container/Tube: 1.5- to 2-mL tube
Specimen Volume: Entire specimen
Collection Instructions:
1. Label specimen as extracted DNA
2. Provide indication of volume of DNA.
Specimen Stability Information: Frozen (preferred)/ Refrigerate/Ambient
Additional Information: We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed, and a charge will be applied.
Specimen Type: Paraffin-embedded bone marrow aspirate clot
Container/Tube: Paraffin block
Specimen Stability Information: Ambient
Specimen Type: Tissue (FFPE)
Container/Tube: Paraffin block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block.
Specimen Stability Information: Ambient
Additional Information: Decalcified core biopsies are not accepted.
Specimen Minimum Volume
Blood, bone marrow: 1 mL; Extracted DNA: 50 mcL at 10 ng/mcL concentration; Paraffin block: 1 block
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | Reject |
| Moderately to severely clotted Bone marrow core biopsies (decalcified embedded) Slides Paraffin shavings | Reject |
Special Instructions
Day(s) Performed
Monday through Friday
Report Available
4 to 7 daysMethod Name
Droplet Digital Polymerase Chain Reaction (ddPCR)
Reporting Name
KIT D816V Variant Analysis Quant, VPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Secondary ID
620901CPT Code Information
81273
Reference Values
An interpretive report will be provided indicating the status as positive or negative for KIT p.Asp816Val (D816V).
KIT gene (NCBI accession NM_000222.3)
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.Forms
SANFORD LABORATORY INTERFACE BUILD INFORMATION
| Result Code | Result Code Description |
|---|---|
| 28288 | Specimen Type |
| 28289 | Interpretation |
| 28290 | Signing Pathologist |