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Test ID BLOD1483 Hypercoagulable Panel - Steady State, No Anticoag Therapy

Specimen Type/Requirements

Lavender top (EDTA) tube - Whole Blood

and

Blue top (Sodium Citrate) tube - Plasma, Double Spun Platelet Poor (14 separate frozen aliquots)

 

See Coagulation Testing and Platelet Poor Plasma Preparation Protocol

 

Note:  EDTA tubes that have been aliquoted or pierced with instrumention probes will not be accepted.  Patients receiving heparin therapy should discontinue medication for 2 days before testing.

 

Testing is affected by hemolysis and lipemia. 

Specimen Volume

EDTA Whole Blood:

 Preferred Volume     4.0 mL   
 Minimum Volume     200 uL   

Sodium Citrate Plasma (14 frozen aliquots):

 Preferred Volume     17.0 mL (11 - 1.0 mL aliquots and 3 - 2.0 mL aliquots   
 Minimum Volume     11.0 mL (1 - 1.5 mL aliquot and 6 - 1.0 mL aliquots and 7 - 0.5 mL aliquots   

 

Stability/Transport

EDTA Whole Blood:

 Room Temperature     24 hours     
 Refrigerated     7 days     Preferred for transport   
 Frozen     12 months     

Sodium Citrate Plasma:

 Room Temperature     Not Acceptable     
 Refrigerated     Not Acceptable     
 Frozen     14 days     Preferred for transport   

 

Performed Test Frequency

Varies depending on performing lab schedule

Additional Information

Test includes: Protein C Functional Activity, Protein S Functional Activity, Cardiolipin IgG Antibody, Cardiolipin IgM Antibody, Pt G20210A Gene Mutation, Factor V (5) Leiden Mutation Analysis, Antithrombin 3, D-Dimer Quantitative, Fibrinogen, Protime, Dilute Russel Viper Venon Time, PTT, Beta 2 Glycoprotein I IgG, Beta 2 Glycoprotein I IgM, and Lupus Inhibitor Screen (Intrinsic Pathway).

 

Abnormal results can be obtained in patients on anticoagulant therapy, including the newer agents (e.g. rivaroxaban, apixaban, etc.) This can result in false interpretation of lupus inhibitor.

Methodology

Varies, See individual tests for method

Performing Lab

Sanford Fargo and Sanford Sioux Falls

CPT

85303, 85306, 86147 (2), 81240, 81241, 85300, 85610, 85379, 85384, 85613, 85730, 86146 (2), 85598