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Test ID BLOD1390 Testosterone, Free (Dialysis) and Total, MS

Important Note

Early morning testosterone levels in young male individuals are, on average, 50% higher than p.m. levels. Reference values were established using specimens collected in the morning.

 

Effective 3/21/2025:  Due to reagent issues affecting Mayo testing, results will be delayed past the published turnaround time. Specimens will be stabilized upon arrival and processed in the order they are received. Specimens may take up to 12 days to result and the duration of the delay is expected to last up to 60 days or more. An update will be provided when standard testing resumes.


Specimen Required


Collection Container/Tube:

Preferred:  Red top

Acceptable: Serum gel

Submission Container/tube: Plastic vial

Specimen Volume: 1.8 mL

Collection Instructions:

1. Allow blood to clot at room temperature. Centrifuge and immediately aliquot 1.8 mL of serum into a plastic vial.

2. Send refrigerate

Specimen Stability Information: Refrigerated (preferred) 21 days/Ambient 7 days/Frozen 2 years


Secondary ID

75751

Useful For

Helpful in assessing testicular function in prepubescent hypogonadal males and in managing hirsutism and virilization in females

Method Name

Chromatography/Mass Spectrometry, Equilibrium Dialysis, Calculation (CALC)

Reporting Name

Testosterone Free and Total

Specimen Type

Serum Red

Specimen Minimum Volume

0.9 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 21 days
  Ambient  7 days
  Frozen 

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

TESTOSTERONE, TOTAL, MS

Age

Males (ng/dL)

Females (ng/dL)

Cord blood

17-61

16-44

1-10 days

≤187

≤24

11-30 days

Not established

Not established

1-2 months

72-344

≤17

3-4 months

≤201

≤12

5-6 months

≤59

≤13

7-11 months

≤16

≤11

1-5 years

≤5

≤8

6-7 years

≤25

≤20

8-10 years

≤42

≤35

11 years

≤260

≤40

12-13 years

≤420

≤40

14-17 years

≤1000

≤40

≥ 18 years

250-1100

2-45

 

TESTOSTERONE, FREE

Age

Males (pg/mL)

Females (pg/mL)

0-4 years

Not established

Not established

5-9 years

<5.4

0.2-5.0

10-13 years

0.7-52.0

0.1-7.4

14-17 years

18.0-111.0

0.5-3.9

18-69 years

35.0-155.0

0.1-6.4

70-89 years

30.0-135.0

0.2-3.7

>89 years

Not established

Not established

Day(s) Performed

Monday through Sunday

Report Available

6 to 9 days

Performing Laboratory

Quest Diagnostics Nichols Institute

Test Classification

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

CPT Code Information

84402

84403