Test ID BLOD0200 Alkaline Phosphatase, Total and Isoenzymes, Serum
Useful For
Aid in the diagnosis and treatment of liver, bone, intestinal, and parathyroid diseases
Determining the tissue source of increased alkaline phosphatase (ALP) activity in serum
Differentiating between liver and bone sources of elevated ALP
Specimen Type
SerumNecessary Information
Patient's age and sex are required.
Specimen Required
Patient Preparation: Fasting (8 hours) required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Within 2 hours of collection, centrifuge the specimen.
2. For red top tubes, immediately aliquot into a plastic vial.
3. For serum gel tubes, serum may sit on gel refrigerated but must be aliquoted within 7 days.
Specimen Minimum Volume
1 mL
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Serum | Frozen (preferred) | 14 days |
Ambient | 7 days | |
Refrigerated | 7 days |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | OK |
Gross icterus | Reject |
Day(s) Performed
Tuesday through Saturday
Report Available
3 to 5 daysMethod Name
ALP: Colorimetric
ALPI: Electrophoresis
Reporting Name
Alkaline Phosphatase, Tot and Iso,SPerforming Laboratory

Secondary ID
622157CPT Code Information
84075
84080
Reference Values
ALKALINE PHOSPHATASE
Males
0-14 days: 83-248 U/L
15 days-<1 year: 122-469 U/L
1-<10 years: 142-335 U/L
10-<13 years: 129-417 U/L
13-<15 years: 116-468 U/L
15-<17 years: 82-331 U/L
17-<19 years: 55-149 U/L
≥19 years: 40-129 U/L
Females
0-14 days: 83-248 U/L
15 days-<1 year: 122-469 U/L
1-<10 years: 142-335 U/L
10-<13 years: 129-417 U/L
13-<15 years: 57-254 U/L
15-<17 years: 50-117 U/L
≥17 years: 35-104 U/L
ALKALINE PHOSPHATASE ISOENZYMES
Ages:
≤17 years: Refence values have not been established for patients less than 18 years of age.
≥18 years:
Liver %: 30.2-74.7
Liver U/L: 15.8-71.9
Bone %: 23.8-68.3
Bone U/L: 12.0-56.7
Intestine %: ≤22.5
Intestine U/L: ≤12.6
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
ALP | Alkaline Phosphatase, S | Yes | Yes |
ALPI | Alkaline Phosphatase Isoenzymes, S | No | Yes |
SANFORD LABORATORY INTERFACE BUILD INFORMATION
Result Code | Result Code Description |
---|---|
17880 | Alkaline Phosphatase |
17718 | Alkaline Phosphatase Isoenzymes |
28728 | Liver % |
28729 | Liver |
17723 | Bone % |
17724 | Bone |
17725 | Intestine % |
17726 | Intestine |