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HelpTest ID BLOD0119 Osmotic Fragility, Erythrocytes
Useful For
Evaluating suspected hereditary spherocytosis-associated hemolytic anemia
Confirming or detecting mild spherocytosis
Specimen Type
ControlWhole Blood EDTA
Ordering Guidance
Shipping Instructions
Specimens must arrive within 72 hours of collection.
Necessary Information
Patient's age is required.
Specimen Required
Both patient and shipping control whole blood specimens are required. For complete instructions, refer to Specimen Collection and Labeling Instructions for Osmotic Fragility Testing of Erythrocytes.
Shipping Control Specimen
Specimen Type: Whole blood (non-patient)
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Collect a shipping control specimen from a healthy person unrelated to the patient at the same time (or within 4 hours) as the patient specimen.
Note: The shipping control specimen can be collected from a phlebotomist, volunteer or another heathy patient.
2. Clearly handwrite "CONTROL" on the outermost label of the tube.
3. Immediately refrigerate (or place on cold gel pack/small amount of wet ice) after collection.
4. Send shipping control in the original tube. Do not aliquot.
5. Keep the shipping control and the patient specimens together, either rubber banded or in a bag.
Additional Information:
1. The shipping control and patient specimens must be handled identically from the time of collection through receipt in the testing laboratory.
2. If the shipping control is not sent with the patient specimen, test cancellation is likely.
3. The shipping control specimen evaluates whether the patient result has been compromised by handling conditions such as temperature, motion, or other transportation interferences, as temperature and handling extremes can adversely impact the integrity of the specimen.
Patient Specimen
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Collect and label patient specimen.
2. Immediately refrigerate (or place on cold gel pack/small amount of wet ice) specimen after collection.
3. Send whole blood specimen in the original tube. Do not aliquot.
4. Keep the shipping control and the patient specimens together, either rubber banded or in a bag.
Additional Information: The shipping control and patient specimens must be handled identically from the time of collection through receipt in the testing laboratory.
Reject Due To
| Gross hemolysis | Reject |
| Clotted blood | Reject |
Specimen Minimum Volume
Patient whole blood, shipping control: 2 mL each
Specimen Stability Information
| Specimen Type | Temperature | Time | |
|---|---|---|---|
| Control | Refrigerated | 72 hours | PURPLE OR PINK TOP/EDTA |
| Whole Blood EDTA | Refrigerated | 72 hours |
Day(s) Performed
Monday through Saturday
Report Available
2 to 5 daysMethod Name
Osmotic Lysis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
85557
Reporting Name
Osmotic Fragility, RBCReference Values
≥12 months:
0.50 g/dL NaCl (unincubated): 3-53% hemolysis
0.60 g/dL NaCl (incubated): 14-74% hemolysis
0.65 g/dL NaCl (incubated): 4-40% hemolysis
0.75 g/dL NaCl (incubated): 1-11% hemolysis
NaCl = Sodium chloride
Reference values have not been established for patients who are younger than 12 months
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.
Special Instructions
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| FRAGO | Osmotic Fragility | No | Yes |
| SCTRL | Shipping Control Vial | No | Yes |
Secondary ID
9064SANFORD LABORATORY INTERFACE BUILD INFORMATION
| Result Code | Result Code Description |
|---|---|
| 13662 | Osmotic Fragility |
| 13663 | OS FR 0.50 G/DL NACL |
| 13664 | OS FR 0.60 G/DL NACL |
| 13665 | OS FR 0.65 G/DL NACL |
| 13666 | OS FR 0.75 G/DL NACL |
| 13667 | Osmotic Fragility Comment |
| 13668 | Shipping Control Vial |