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Test ID NBLD0664 Alzheimer Disease Evaluation, Spinal Fluid


Specimen Required


Supplies:

Alzheimer's Disease Evaluation (ADEVL) Collection Kit (T836)

CSF AD Biomarker Tubes (T833; also included in T836)

Container/Tube:

Preferred: Sarstedt CSF False Bottom Tube 63.614.625 (2.5 mL)

Acceptable: Sarstedt 72.703.600 (1.5 mL) or Sarstedt 72.694.600 (2 mL)

Specimen Volume: 1.5 to 2.5 mL

Collection Instructions:

1. Perform lumbar puncture and discard the first 1 to 2 mL of cerebrospinal fluid (CSF).

2. Collect CSF directly into one of the listed collection tubes until the tube is at least 50% full.*

3. Send CSF specimen in original collection tube. Do not aliquot.

Note: Polystyrene collection tubes are not acceptable. Exposure of CSF to polystyrene tubes may result in falsely low Abeta42 concentrations. For more information see Cautions.

*The Alzheimer's Association consensus protocol for handling of CSF for clinical measurements of Abeta42 and tau recommends using the drip method for CSF collection and directly collecting into a low-bind polypropylene tube. Although some clinicians prefer the syringe pull method due to speed of collection, the drip method reduces the risk of Abeta42 binding to the plastic of any syringe used.

4. Collection instructions can also be found on Spinal Fluid Specimen Collection Instructions for Alzheimer Disease Evaluation (T967).


Sanford Laboratories - Sioux Falls/Rapid City Note:

Note: Picture of Alzheimer's Disease Evaluation (ADEVL) Collection Kit at bottom of page.

Secondary ID

607273

Useful For

Assessment of adults with cognitive impairment being evaluated for Alzheimer disease and other causes of cognitive impairment

 

These assays should not be used to predict the development of dementia or other neurologic conditions or to monitor response to therapies.

Method Name

Electrochemiluminescent Immunoassay (ECLIA)

Reporting Name

Alzheimer's Disease Evaluation, CSF

Specimen Type

CSF

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 14 days BlueTop SARSTEDT
  Frozen  60 days BlueTop SARSTEDT
  Ambient  12 hours BlueTop SARSTEDT

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus Reject

Reference Values

Beta-amyloid (1-42) (Abeta42): >834 pg/mL

 

Total-Tau: ≤238 pg/mL

 

Phosphorylated-Tau 181: ≤21.6 pg/mL

 

p-Tau/Abeta42: ≤0.028

Day(s) Performed

Tuesday, Thursday, Friday

Report Available

1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83520 x 3

Forms

If not ordering electronically, complete, print, and send a Neurology Specialty Testing Client Test Request (T732) with the specimen.

Sanford Laboratories - Sioux Falls/Rapid City Additional Information:

 Alzheimer's Disease Evaluation (ADEVL) Collection Kit 

 

SANFORD LABORATORY INTERFACE BUILD INFORMATION

 Result Code     Result Code Description   
 27330  P-TAU/ABETA-42
 27331  Ad Interpretation
 27332  ABETA42
 27333  Total-TAU
 27334  Phospho-TAU(181P)