Test ID NBLD0487 EGFR Gene, Targeted Mutation Analysis, 51 Mutation Panel, Tumor
Specimen Type
VariesNecessary Information
Pathology report must accompany specimen for testing to be performed.
Specimen Required
Preferred: Submit 3, if available, or 2 of the following specimens.
Acceptable: Submit at least one of the following specimens.
Specimen Type: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block with acceptable amount of tumor tissue.
Specimen Type: Tissue slide
Source: FFPE tissue, cell block (prepared from fine-needle aspirate, or pleural fluid)
Slides: 1 Hematoxylin and eosin-stained and 5 unstained
Collection Instructions:
Submit the followings slides:
1 Slide stained with hematoxylin and eosin
AND
5 Unstained, nonbaked slides with 5-micron thick sections of the tumor tissue.
Specimen Type: Cytology slide (direct smears; smears stained with Pap stain are not acceptable)
Slides: 2 Slides
Collection Instructions: Submit 2 slides stained with Diff Quik and coverslipped with a total of 5000 nucleated cells (preferred) or at least 3000 nucleated cells (minimum).
Note: Glass coverslips are preferred; plastic coverslips are acceptable but will result in longer turnaround times.
Additional Information: Cytology slides will not be returned. An image of the slides will be stored per regulatory requirements.
Specimen Minimum Volume
Formalin-fixed, paraffin-embedded tissue block or Slides: see Specimen Required
Cytology smear stained with Diff Quik: >1000 cells
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Varies | Ambient (preferred) | |
Refrigerated |
Reject Due To
Specimens that have been decalcified (all methods) | Reject |
Day(s) Performed
Monday through Friday
Report Available
4 to 7 daysReporting Name
EGFR Gene, Mutation Analysis, TumorMethod Name
Polymerase Chain Reaction (PCR)
Performing Laboratory

CPT Code Information
81235-EGFR (epidermal growth factor receptor) (eg, non-small cell lung cancer) gene analysis, common variants (eg, exon 19 LREA deletion, L858R, T790M, G719A, G719S, L861Q)
88381-Microdissection, manual
Secondary ID
614665Useful For
Identifying non-small cell lung cancers that may respond to epidermal growth factor receptor-targeted therapies
Additional Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
SLIRV | Slide Review in MG | No, (Bill Only) | Yes |
Testing Algorithm
When this test is ordered, slide review will always be performed at an additional charge.
Reference Values
An interpretive report will be provided.
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.
SANFORD LABORATORY INTERFACE BUILD INFORMATION
Result Code | Result Code Description |
---|---|
19247 | Result Summary |
19248 | Result |
19249 | Interpretation |
19250 | Specimen |
19251 | Source |
19252 | Tissue ID |
19253 | Released By |