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HelpTest ID NBLD0433 Plasma Cell DNA Content and Proliferation, Bone Marrow
Useful For
Establishing a diagnosis of a plasma cell proliferative disorder
Providing prognostic information for newly diagnosed multiple myeloma and other plasma cell proliferative disorders
Assessing response to therapy and detecting disease relapse and progression in treated plasma cell proliferative disorder patients
Determining plasma cell DNA content and proliferation
Specimen Type
Bone MarrowOrdering Guidance
This test can be ordered at diagnosis or follow-up of a plasma cell neoplasm (plasma cell proliferative disorder).
If CSMRT / mSMART Plasma Cell Proliferative Disorder, Pre-Analysis Cell Sorting, Bone Marrow or MPCDS / mSMART, Plasma Cell Proliferative Disorder, FISH, Bone Marrow is desired to be performed at Mayo, order MSMRT / Mayo Algorithmic Approach for Stratification of Myeloma and Risk-Adapted Therapy Report, Bone Marrow.
The Multiple Myeloma: Laboratory Screening algorithm will allow plasma cell fluorescence in situ hybridization (FISH) testing to be added, based on this test's flow cytometry results.
Necessary Information
1. Include patient's disease state (untreated, treated, monoclonal gammopathy of undetermined significance, stable).
2. Indicate if patient is on anti-CD38 therapy.
Specimen Required
Specimen Type: Redirected bone marrow
Preferred: Yellow top (ACD solution A or B)
Acceptable: Lavender top (EDTA) or green top (sodium heparin)
Specimen Volume: 4 mL
Specimen Stability Information: 3 days
Reject Due To
| Gross hemolysis | Reject |
| Fully clotted | Reject |
Specimen Minimum Volume
2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Bone Marrow | Ambient (preferred) | ||
| Refrigerated | |||
Day(s) Performed
Preanalytical processing: Monday through Saturday
Results reported: Monday through Friday
Report Available
1 to 4 daysMethod Name
Flow Cytometry/DNA Content/Cell Cycle Analysis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
88182-Flow cytometry, cell cycle or DNA analysis
88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker
88185 x 5-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker (each)
88187-Flow cytometry interpretation, 2 to 8 Markers (added as FCINT)
Reporting Name
Plasma Cell Proliferation, MarrowReference Values
Plasma Cell Clonality:
Normal bone marrow
No monotypic clonal plasma cells detected
DNA Index:
Normal polytypic plasma cells
DNA index (G0/G1 cells): Diploid 0.95-1.05
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Special Instructions
Testing Algorithm
When this test is ordered, flow cytometry interpretation will always be performed at an additional charge.
For more information see:
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| FCINT | Flow Cytometry Interp, 2-8 Markers | No, (Bill Only) | Yes |
Secondary ID
61654SANFORD LABORATORY INTERFACE BUILD INFORMATION
| Result Code | Result Code Description |
|---|---|
| 15880 | Monotypic Plasma Cells: |
| 15881 | Monotypic PC Per Total Events |
| 15882 | Monotypic Plasma Cells S-Phase |
| 15883 | Monotypic Plasma Cells DNA Index |
| 15884 | Monotypic Plasma Cells DNA Ploidy |
| 15885 | Polytypic PC Per Total Events |
| 15886 | Polytypic PC Per All Plasma Cells |
| 15887 | Final Diagnosis |