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HelpTest ID NBLD0416 Plasma Cell Proliferative Disorder, High Risk with Reflex Probes, Diagnostic FISH Evaluation, Bone Marrow
Useful For
Aiding in the diagnosis of new cases of multiple myeloma or other plasma cell proliferative disorders using bone marrow specimens
Identifying prognostic markers based on the abnormalities found
This test should not be used to track the progression of disease.
Specimen Type
Bone MarrowOrdering Guidance
For a more complete genetic evaluation, order MPCDS / mSMART, Plasma Cell Proliferative Disorder, FISH, Bone Marrow.
For testing paraffin-embedded tissue samples from patients with a plasma cell disorder, order PLASF / Plasma Cell Proliferative Disorder, FISH, Tissue.
For fixed cell pellet specimens, order MFCDF / Myeloma, High Risk, with Reflex Probes, Diagnostic FISH Evaluation, Fixed Cell Pellet.
Testing will be changed to the appropriate test if this test is ordered on either of the previous specimens or if bone marrow specimens are received more than 96 hours from collection.
Shipping Instructions
1. Specimen should arrive within 96 hours of collection.
2. Advise Express Mail or equivalent if not on courier service.
Necessary Information
A reason for testing and a flow cytometry and/or a bone marrow pathology report should be submitted with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.
Specimen Required
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Invert several times to mix bone marrow
2, Send bone marrow specimen in original tube. Do not aliquot.
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Specimen Minimum Volume
2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Bone Marrow | Ambient (preferred) | 4 days | |
| Refrigerated | 4 days |
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysMethod Name
PCPDS, PCPDB: Fluorescence In Situ Hybridization (FISH)
CSPCF: Flow Cytometric Cell Selection
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
88271 x 2, 88274, 88291-FISH Probe, Analysis, Interpretation; 1 probe set
88271 x 2, 88274-FISH Probe, Analysis; each additional probe set (if appropriate)
Reporting Name
Plasma Cell Prolif, High Risk, FISHReference Values
An interpretive report will be provided.
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| PCPDB | Probe, Each Additional (PCPDS) | No, (Bill Only) | No |
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CSPCF | PCPDS Pre-Analysis Cell Sorting, BM | No | Yes |
Secondary ID
606079Testing Algorithm
This test is designed for diagnostic specimens from patients with multiple myeloma or other plasma cell proliferative disorders.
When this test is ordered, pre-analysis cell sorting will be performed at an additional charge.
The fluorescence in situ hybridization (FISH) panel includes testing for the following abnormalities using the FISH probes listed:
17p-, TP53/D17Z1
1q gain, TP73/1q22
14q32 rearrangement, IGH break-apart
Based on the results from the initial panel, reflex testing may be performed to identify the following abnormalities using the probes listed:
t(11;14)(q13;q32), CCND1/IGH fusion
t(14;16)(q32;q23), IGH/MAF fusion
t(4;14)(p16.3;q32), FGFR3/IGH fusion
t(14;20)(q32;q12), IGH/MAFB fusion
For follow-up samples, the following probes will be evaluated if sufficient plasma cells are identified:
If a previous diagnostic sample was uninformative for a probe set, attempts may be made to achieve results for the missing probe on a subsequent sample (if sufficient plasma cells are identified).
17p-, TP53/D17Z1
1q gain, TP73/1q22
8q24.1 rearrangement, MYC break-apart
Initial screening will be performed to determine if sufficient plasma cells are present within the provided specimen. If the specimen is received greater than 96 hours from collection, this test will be canceled and MFCDF / Myeloma, High Risk with Reflex Probes, Diagnostic FISH Evaluation, Fixed Cell Pellet will be added as the more appropriate test.
Appropriate ancillary probes may be performed at consultant discretion to render comprehensive assessment. Any additional probes will have the results included within the final report and will be performed at an additional charge.
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
SANFORD LABORATORY INTERFACE BUILD INFORMATION
| Result Code | Result Code Description |
|---|---|
| 19513 | Result Summary |
| 19514 | Interpretation |
| 19515 | Result Table |
| 19516 | Result |
| 19517 | Reason for Referral |
| 19518 | Specimen |
| 19519 | Source |
| 19520 | PCPDF Method |
| 19521 | Additional Information |
| 19522 | Disclaimer |
| 19523 | Released By |