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HelpTest ID MICR0037 Culture Bacterial, Stool with Shiga-Like Toxin
Specimen Type/Requirements
Orange Cap Para-Pak C&S (Cary Blair Transport Media) - Stool (Refer to Stool Collection Patient Instruction link below)
Also acceptable:
Green Cap Para-Pak Enteric Plus (Cary Blair Transport Media) - Stool (Refer to Stool Collection Patient Instruction link below)
Red Cap Enteric Transport Medium (ETM) - Stool (Refer to Stool Collection Patient Instruction link below)
Sterile Container - Stool (only stable 2 hours refrigerated)
Note: Stool that is not in transport media and is received greater than 2 hours after collection may be detrimental to the recovery of Shigella spp.
Rectal swabs should be placed in Cary Blair Media immediately.
Please reference links for patient instructions:
Specimen Volume
| Preferred Volume | 2.0 grams |
|---|
Stability/Transport
Orange Cap Para-Pak C&S; Green Cap Para-Pak Enteric Plus (Cary Blair Transport Media) or Red Cap Enteric Transport Medium (ETM):
| Room Temperature | Not Acceptable | |
|---|---|---|
| Refrigerated | 24-72 hours* | Preferred for transport |
| Frozen | Not Acceptable |
*Refrigerated Cary Blair samples should be processed within 24 hours for best recovery of pathogens.
Steile Container:
| Room Temperature | Not Acceptable | |
|---|---|---|
| Refrigerated | 2 hours | Preferred for transport |
| Frozen | Not Acceptable |
Performed Test Frequency
Monday through Friday. Positives are reported the same day as set up. Negatives are “ENHANCED” and set up again the next day.
Additional Information
Test includes: Stool Culture (Includes Salmonella/Shigella, Campylobacter, E. coli O157, Aeromonas, Vibrio and Plesiomonas) and Rapid Screening for Shiga-Like Toxin 1 And 2 Antigens.
Identification, typing, and/or sensitivity testing will be performed if indicated at an additional fee (per organism).
If stool is positive for E. coli O157, then H7 typing will be performed.
Follow-up cultures on previous positive patients should be ordered as a screen for a specific pathogen (MICR0002).
Methodology
Culture and Enzyme Immunoassay
Performing Lab
Sanford Laboratories Sioux Falls