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Test ID LBOR0172 FLT3 Mutation Analysis, Varies

Useful For

Prognostic indication for some patients with acute myeloid leukemia

 

This test should not be used to monitor residual disease following treatment.

Specimen Type

Varies


Ordering Guidance


This test is intended to be used as a prognostic test at diagnosis and should not be used to monitor residual disease following treatment.



Shipping Instructions


Specimen must arrive within 7 days of collection.



Necessary Information


The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date and time of collection

4. Specimen source



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA) or yellow top (ACD)

Specimen Volume: 3 mL

Collections Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube. Do not aliquot.

3. Label specimen as whole blood.

Specimen Stability Information: Ambient (preferred) 7 days/Refrigerate 7 days

 

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA) or yellow top (ACD)

Specimen Volume: 2 mL

Collections Instructions:

1. Invert several times to mix bone marrow.

2. Send specimens in original tube. Do not aliquot.

3. Label specimen as bone marrow.

Specimen Stability Information: Ambient (preferred) 7 days/Refrigerate 7 days

 

Specimen Type: Extracted DNA from whole blood or bone marrow

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions:

1. DNA must be extracted from blood or bone marrow within 7 days of collection

2. Label specimen as extracted DNA and source of specimen

2. Provide volume and concentration of DNA on the label.

Specimen Stability Information: Frozen (preferred)/Refrigerate/Ambient

Additional Information: DNA must be extracted in a CLIA-certified laboratory or equivalent and must be extracted from a specimen type listed as acceptable for this test (including applicable anticoagulants). We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed and a charge will be applied.


Reject Due To

Gross hemolysis Reject
Bone marrow biopsies Reject
Slides Reject
Paraffin shavings Reject
Moderately to severely clotted Reject

Specimen Minimum Volume

Whole blood, Bone marrow: 1 mL; Extracted DNA: 50 mcL at 20 ng/mcL concentration

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies 7 days

Day(s) Performed

Monday through Saturday

Report Available

3 to 6 days

Method Name

Polymerase Chain Reaction (PCR)/Capillary Electrophoresis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81245-FLT3 (fms-related tyrosine kinase) (eg, acute myeloid leukemia), gene analysis, internal tandem duplication (ITD) variants (ie, exons 14, 15)

81246-FLT3 (fms-related tyrosine kinase 3) (eg, acute myeloid leukemia), gene analysis; tyrosine kinase domain (TKD) variants (eg, D835, I836)

Reporting Name

FLT3 Mutation Analysis, V

Reference Values

An interpretive report will be provided.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Secondary ID

19739
Sanford Laboratories - Enterprise Additional Information:

SANFORD LABORATORY INTERFACE BUILD INFORMATION

Result Code  Result Code Description 
17618 Specimen
11515 FLT3 Mutations (ITD and D835) 
17622 Final Diagnosis