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Test ID LBOR0021 Enterovirus RNA, Qualitative by PCR

Important Note

Per performing lab at Quest Chantilly as of 4/3/2018, change has been made to instructions.

Specimens collected that are placed in Universal Transport Media (UTM) must have the swab left in the media and will be sent frozen as indicated below.

Specimen Type/Requirements

Lavender top (EDTA) tube -  Plasma
Yellow top (ACD-A or ACD-B) tube -  Plasma
Gold top (Serum w/ gel) tube - Serum

Red top (Serum w/out gel) tube - Serum

Sterile Container - Stool, CSF, Pericardial Fluid


UTM or M4 media - Swab from throat, rectal, lesion or nasopharyngeal source

 

Separate plasma/serum from cells within 2 hours of collection.

Leave swab in UTM/M4 media.

Indicate source on specimen and requisition.

Test is not affected by hemolysis or lipemia. 

Specimen Volume

Preferred Volume (plasma/serum/CSF, Pericardial Fluid)   0.7 mL  
Preferred Volume (stool)   1.0 g  
Minimum Volume (plasma/serum/CSF, Pericardial Fluid)   0.3 mL  
Minimum Volume  (stool)   0.5 g

 

Stability/Transport

 

  Room Temperature   Refrigerated   Frozen   Preferred for transport  
Plasma/Serum/CSF/Pericardial Fluid/Transport Media 48 hours   7 days  

30 days

 Frozen
Stool unacceptable   unacceptable   30 days  Frozen

 

Performed Test Frequency

Monday through Sunday

Report Available

1 day

Additional Information

This test includes coxsackie, polio and echoviruses but does not differentiate.

Methodology

Real-Time Polymerase Chain Reaction

Performing Lab

Quest Chantilly

CPT

87498

Interface Build Information

Result Code Result Code Description
15993 Source
27443 Enterovirus RNA, RT-PCR