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Test ID BLOD1681 Protoporphyrins, Fractionation, Whole Blood

Reporting Name

Protoporphyrins, Fractionation, WB

Useful For

Evaluating patients with possible diagnoses of erythropoietic protoporphyria or X-linked dominant protoporphyria

 

Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria

Testing Algorithm

This test should not be ordered in conjunction with PEE / Porphyrins Evaluation, Whole Blood.

 

The following information is available:

-Porphyria (Acute) Testing Algorithm

-Porphyria (Cutaneous) Testing Algorithm

-The Heme Biosynthetic Pathway

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole blood


Ordering Guidance


This test is for assessment for protoporphyria. The preferred test for lead toxicity in children is blood lead. For more information see PBDV / Lead, Venous with Demographics, Blood or PBDC / Lead, Capillary, with Demographics, Blood. The preferred screening test for suspicion of a hepatic porphyria is urine porphyrins. For more information see PQNRU / Porphyrins, Quantitative, Random, Urine. This test should not be ordered with PEE / Porphyrins Evaluation, Whole Blood



Necessary Information


Include a list of medications the patient is currently taking.



Specimen Required


All porphyrin tests on whole blood can be performed on 1 tube.

 

Patient Preparation: Patient must not consume any alcohol for 24 hours before specimen collection.

Container/Tube:

Preferred: Green top (sodium heparin)

Acceptable: Dark blue top (metal free heparin), green top (lithium heparin), or lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions: Refrigerate specimen as soon as possible.


Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Refrigerated 7 days

Reference Values

FREE PROTOPORPHYRIN

<20 mcg/dL

 

ZINC-COMPLEXED PROTOPORPHYRIN

<60 mcg/dL

Day(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82542

Report Available

3 to 5 days

Reject Due To

Gross hemolysis Reject

Method Name

High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection

Secondary ID

8739
Sanford Laboratories - Sioux Falls/Rapid City Additional Information:

SANFORD LABORATORY INTERFACE BUILD INFORMATION

 Result Code     Result Code Description   
 27113  Zinc-Complexed Protophorphyrin   
 27114  Free Protoporphyrin
 6358  Interpretation