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Test ID BLOD0661 Factor VIII (8) Assay

Useful For

Diagnosing Hemophilia A; Diagnosing von Willebrand Disease when measured with the von Willebrand Factor (VWF) Antigen and VWF Activity; Diagnosing aquired deficiency states; Investigation of prolonged Activated Partial Thromboplastin Time; Monitoring infusions of Factor VIII replacement during interventional procedures and prophylactic infusions.

This test is indicated for Hemophilia patients being treated with Advate therapy. 

 

Ordering Information:  

Note: If the patient is on any of the following medications for Hemophilia A or B (see list below).  This test (BLOD0661) is not appropriate and should not be ordered.  Order instead, Chromogenic Factor VIII Activity Assay (BLOD1653).

  Medications Requiring Chromogenic Assays (BLOD1653)*  
   Hemlibra (Emicizumab)   
   Helixate (Afstyla)
Jivi
Adynovate
Eloctate
Novo Eight
Nuwig
Esperoct
Refacto

*There may be other extended half-life medication treatments for Hemophilia A or B not included in the list that may require the Chromogenic Factor VIII Activity Assay (BLOD1653).

 

Note:  If patient is on any of the above treatment therapies, or other Hemophilia medications, indicate such information with the order:

  • Is the patient receiving treatment for Hemophilia A or B?  Yes or No
  • If Yes, list medication. 

Specimen Type/Requirements

Blue top (Sodium Citrate) tube - Double Spun Platelet Poor Plasma

 

See Coagulation Testing and Platelet Poor Plasma Preparation

 

This test requires its own separate frozen aliquot.  The aliquot must be from an appropriately filled 3.2% Sodium Citrate tube to acquire the 9:1 blood to anticoagulant ratio that is critical for valid results.

 

Platelet poor plasma should be frozen within 2 hours of collection.  Double spun (platelet poor plasma) is critical for accurate results. 

 

Patient must not be receiving Heparin or Coumadin Therapy.

 

Test is affected by moderate hemolysis.  Test is not affected by lipemia.

Specimen Volume

 Preferred Volume     1.0 mL   
 Minimum Volume     0.5 mL   

 

Stability/Transport

 Room Temperature     2 hours     
 Refrigerated     4 hours     
 Frozen     1 month     Preferred for transport   

 

Performed Test Frequency

Monday through Friday

Methodology

Activated Partial Thromboplastin Clot-Based Assay

Performing Lab

Sanford Laboratories Sioux Falls

CPT

85240