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Test ID BLOD0122 Platelet Antibody Screen, Serum

Useful For

Detecting alloantibodies to epitopes on platelet glycoproteins IIb/IIIa, Ib/Ix, Ia/IIa, IV and class I human leukocyte antigens to evaluate cases of immune mediated refractoriness to platelet transfusions, posttransfusion purpura, or neonatal alloimmune thrombocytopenia

Specimen Type

Serum Red


Ordering Guidance


For neonate testing, consider sending a maternal specimen instead of a neonate specimen as unbound platelet antibodies may not be detected in the neonate serum.

 

This test is not recommended for the diagnosis of immune thrombocytopenia or autoimmune thrombocytopenia. Tests that are optimized to detect antibodies bound to the platelets will be useful in these situations; cell-bound platelet antibody (direct) test is strongly recommended.



Necessary Information


If ordering electronically, answer all prompt questions for timely result reporting:

1. Reason for request is required for result interpretation.

 a. Use provided diagnosis options if appropriate. If specific diagnosis is unknown, select the generic answer of alloimmune thrombocytopenia.

 b. Record only the diagnosis pertaining to this test.

 c. Record diagnosis description instead of code.

2. Indicate if patient has had intravenous immunoglobulin (IVIg) therapy in the last month: Yes or No



Specimen Required


Patient Preparation: Do not collect within 72 hours of a platelet transfusion. Transfused platelets will interfere with this assay.

Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Reject Due To

Gross hemolysis Reject

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Frozen (preferred) 365 days
  Refrigerated  48 hours

Day(s) Performed

Monday through Friday

Report Available

2 to 4 days

Method Name

Solid Phase Enzyme-Linked Immunosorbent Assay (ELISA)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86022

Reporting Name

Platelet Ab Screen, S

Reference Values

Not applicable

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

Testing Algorithm

For more information see Platelet Antibody Testing Algorithm.

Secondary ID

35794
Sanford Laboratories - Sioux Falls/Rapid City Additional Information:

SANFORD LABORATORY INTERFACE BUILD INFORMATION

Result Code  Result Code Description 
20688 Overall Result
20689 Interpretation
20690 GPIIB/IIA (Cell-1)
20691 GPIIB/IIA (Cell-2)
20692 GPIA/IIA (Cell-1)
20693 GPIA/IIA (Cell-2)
20694 GPIB/IX
20695 GPIV
20696 HLA Class I
20697 Provided Patient Diagnosis?
20698 IVIG in Last Month?
20699 Plt Transfusion in Last 72 Hours?
20700 Platelet Count X 10(9)/L