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HelpTest ID BDSP0023 Allo-isoleucine, Blood Spot
Reporting Name
Allo-isoleucine, BSUseful For
Evaluation of newborn screening specimens that test positive for branched-chain amino acids elevations
Follow-up of patients with maple syrup urine disease
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole bloodSpecimen Required
Supplies:
-Card-Blood Spot Collection (Filter Paper) (T493)
-Card-Postmortem Screening (Filter Paper) (T525)
Container/Tube:
Preferred: Blood Spot Collection (Filter Paper)
Acceptable: Local newborn screening card, Whatman Protein Saver 903 filter paper, PerkinElmer 226 filter paper, Munktell filter paper, Postmortem Screening Card
Specimen Volume: 2 Blood spots
Collection Instructions:
1. Do not use device or capillary tube containing EDTA to collect specimen.
2. At least 1 spot should be complete and unpunched.
3. An alternative blood collection option for a patient older than 1 year is a fingerstick. For detailed instructions, see How to Collect a Dried Blood Spot Sample.
4. Include type of feeding information on the collection card.
5. Let blood dry on filter paper at ambient temperature in a horizontal position for a minimum of 3 hours.
6. Do not expose specimen to heat or direct sunlight.
7. Do not stack wet specimens.
8. Keep specimen dry.
Additional Information:
1. For collection instructions see Blood Spot Collection Instructions.
2. For collection instructions in Spanish, see Blood Spot Collection Card-Spanish Instructions (T777).
3. For collection instructions in Chinese, see Blood Spot Collection Card-Chinese Instructions (T800).
Specimen Minimum Volume
1 Blood spot
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole blood | Ambient (preferred) | 59 days | FILTER PAPER |
| Frozen | 59 days | FILTER PAPER | |
| Refrigerated | 59 days | FILTER PAPER |
Reference Values
Allo-isoleucine: <4 nmol/mL
Leucine: 52-269 nmol/mL
Isoleucine: 22-167 nmol/mL
Valine: 84-414 nmol/mL
An interpretive report will also be provided.
Day(s) Performed
Monday through Friday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82136
Report Available
2 to 4 daysReject Due To
| Blood spot specimen that shows layering | Reject |
| Blood spot specimen that shows serum rings | Reject |
| Blood spot specimen that shows multiple applications | Reject |
| Blood spot specimen that shows insufficient specimen | Reject |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Portions of this test are covered by patents held by Quest Diagnostics
Special Instructions
Secondary ID
88888Forms
If not ordering electronically, complete, print, and send a Biochemical Genetics Test Request (T798) with the specimen.
SANFORD LABORATORY INTERFACE BUILD INFORMATION
| Result Code | Result Code Description |
|---|---|
| 26624 | Specimen |
| 26625 | Specimen ID |
| 26626 | Source |
| 26627 | Order Date |
| 26628 | Reason for Referral |
| 26629 | Method |
| 26630 | Allo-Isoleucine |
| 26631 | Leucine |
| 26632 | Isoleucine |
| 26633 | Valine |
| 26634 | Interpretation |
| 26635 | Amendment |
| 26636 | Reviewed By |
| 26637 | Release Date |