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HelpTest ID NBLD0712 Bacterial PCR reflex NGS
Specimen Type/Requirements
Tissue, Fluid, Culture - sterile specimen container, swab (eSwab, UTM), body fluid/bone marrow in EDTA
Acceptable specimen sources: CSF, pleural fluid, pericardial fluid, urine, bronchial lavage, joint fluid, bone marrow, vitreous fluid
Formalin Fixed Paraffin-embedded tissues (FFPE, PET) can be sent ambient or with ice packs during warmer summer months to prevent melting.
Unacceptable specimens: Sputum, blood, serum, plasma, stool/rectal swabs, citrated or heparinized solutions, Tissues floating in formalin, Swab/fluid collected in tube containing agar
Note: We do not need a separate specimen aliquot for each test ordered. Only a single specimen aliquot or block of optimal quantity is necessary for performing multiple tests. If multiple aliquots or blocks of optimal quantity are sent, up to 2 will be pooled.
Please submit: University of Washington Molecular Microbiology Requisition
Specimen Volume
| Preferred Volume |
Fresh Tissue: 0.3-1.0 cm^3 Fluid: 0.2-1.0 mL |
|---|---|
| Minimum Volume |
Fresh Tissue: 0.3-1.0 cm^3 Fluid: 0.2-1.0 mL |
Stability/Transport
| Room Temperature | Not Acceptable | |
|---|---|---|
| Refrigerated | 8 hours | |
| Frozen | Indefinitely | Preferred for transport |
Performed Test Frequency
Monday through Friday
Report Available
Fresh frozen tissues/fluids result in 2-3 business days after receipt of specimen.
Formalin Fixed Paraffin-embedded tissues result in 3-4 business days after receipt of specimen.
Methodology
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
Performing Lab
University of Washington Medical Center
CPT
87801