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HelpTest ID NBLD0711 Acid Fast Bacillus PCR
Specimen Type/Requirements
Tissue, Fluid, Culture - sterile specimen container, swab (eSwab, UTM), body fluid/bone marrow in EDTA
Acceptable specimen sources: CSF, pleural fluid, pericardial fluid, urine, bronchial lavage, sputum, joint fluid, bone marrow, vitreous fluid
Formalin Fixed Paraffin-embedded tissues (FFPE, PET) can be sent ambient or with ice packs during warmer summer months to prevent melting.
Unacceptable specimens: blood, serum, plasma, stool/rectal swabs, citrated or heparinized solutions, tissues floating in formalin, swab/fluid collected in tube containing agar
Note: We do not need a separate specimen aliquot for each test ordered. Only a single specimen aliquot or block of optimal quantity is necessary for performing multiple tests. If multiple aliquots or blocks of optimal quantity are sent, up to 2 will be pooled.
Please submit: University of Washington Molecular Microbiology Requisition
Specimen Volume
| Preferred Volume |
Fresh Tissue: 0.3-1.0 cm^3 Fluid: 0.2-1.0 mL |
|---|---|
| Minimum Volume |
Fresh Tissue: 0.3-1.0 cm^3 Fluid: 0.2-1.0 mL |
Stability/Transport
| Room Temperature | Not Acceptable | |
|---|---|---|
| Refrigerated | 8 hours | |
| Frozen | Indefinitely | Preferred for transport |
Performed Test Frequency
Monday through Friday
Report Available
Fresh frozen tissues/fluids result in 2-3 business days after receipt of specimen.
Formalin Fixed Paraffin-embedded tissues result in 3-4 business days after receipt of specimen.
Additional Information
If sputum sample is submitted, only Mycobacterium avium complex DNA Detection [MAVDNA] and Mycobacterium tuberculosis complex DNA Detection [TBCDNA] will be performed. For other specimen sources, Mycobacterium tuberculosis complex DNA Detection [TBCDNA] and Nontuberculous Mycobacteria DNA detection [NTMDNA] will be performed.
Billing will be driven by the source.
Sputum source: TBCDNA and MAVDNA.
All other sources: TBCDNA and NTMDNA.
Methodology
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
Performing Lab
University of Washington Medical Center
CPT
87551
87556
87561
CPT code billed will be based on specimen source