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Test ID NBLD0270 Amino Acids, Quantitative, Random, Urine

Useful For

Evaluating patients with possible inborn errors of metabolism using random urine specimens

 

May aid in evaluation of endocrine disorders, liver diseases, muscle diseases, neoplastic diseases, neurological disorders, nutritional disturbances, kidney failure, and burns

Specimen Type

Urine


Additional Testing Requirements


Not all patients with homocystinuria will be detected by this assay. If homocystinuria is a concern, order CMMPP / Cobalamin, Methionine, and Methylmalonic Acid Pathways, Plasma or HCYSP / Homocysteine, Total, Plasma or HCYSS / Homocysteine, Total, Serum in tandem with this test.



Necessary Information


1. Patient's age is required.

2. Include family history, clinical condition (asymptomatic or acute episode), diet, and drug therapy information.

3. If prolidase deficiency is a concern, indicate on the amino acid order "Pretreat with acid hydrolysis prior to analysis". The acid hydrolysis will break up in vitro proline and hydroxyproline containing dipeptides, which are cleaved in vivo by prolidase.



Specimen Required


Supplies: Urine Tubes, 10 mL (T068)

Specimen Volume: 2 mL

Collection Instructions: Collect a random urine specimen.


Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Frozen (preferred) 70 days
  Refrigerated  14 days

Day(s) Performed

Monday through Friday

Report Available

3 to 5 days

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Portions of this test are covered by patents held by Quest Diagnostics

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82139

Reporting Name

Amino Acids, QN, Random, U

Reference Values

Amino Acid

Age groups

< 2 months

2-35 months

3-6 years

7-17 years

≥18 years

Taurine

Tau

<9026

<5604

<3680

<3954

<2134

Asparagine

Asn

<687

<1159

<238

<322

<204

Serine

Ser

<3202

<2741

<1386

<1116

<658

Hydroxyproline

Hyp

<2254

<1775

<89

<42

<48

Glycine

Gly

<13336

<9872

<4997

<4467

<4068

Glutamine

Gln

<2931

<3681

<1124

<1038

<764

Aspartic Acid

Asp

<290

<72

<46

<13

<12

Ethanolamine

EtN

<1589

<1765

<776

<536

<500

Histidine

His

<3806

<4578

<2596

<2294

<1508

Threonine

Thr

<1930

<1769

<496

<575

<384

Citrulline

Cit

<61

<67

<23

<17

<34

Sarcosine

Sar

<177

<52

<6

<10

<6

Beta-Alanine

bAla

<174

<79

<99

<139

<58

Alanine

Ala

<2856

<2360

<961

<794

<472

Glutamic Acid

Glu

<281

<210

<127

<63

<62

1-Methylhistidine

1MHis

<493

<470

<517

<453

<265

3-Methylhistidine

3MHis

<196

<662

<3346

<2027

<1920

Argininosuccinic Acid

Asa

<96

<103

<65

<57

<37

Homocitrulline

Hcit

<164

<169

<110

<107

<87

Arginine

Arg

<250

<147

<81

<42

<65

Alpha-aminoadipic Acid

Aad

<264

<397

<349

<138

<73

Gamma Amino-n-butyric Acid

GABA

<8

<14

<4

<3

<4

Beta-aminoisobutyric Acid

bAib

<2214

<2488

<840

<251

<265

Alpha-amino-n-butyric Acid

Abu

<58

<44

<37

<29

<19

Hydroxylysine

Hyl

<101

<87

<44

<22

<16

Proline

Pro

<1776

<1028

<29

<42

<98

Ornithine

Orn

<344

<238

<228

<291

<193

Cystathionine

Cth

<118

<53

<23

<24

<33

Cystine

Cys

<486

<285

<111

<84

<142

Lysine

Lys

<2217

<1321

<814

<463

<295

Methionine

Met

<76

<45

<24

<22

<15

Valine

Val

<308

<258

<119

<91

<73

Tyrosine

Tyr

<453

<603

<289

<371

<155

Isoleucine

Ile

<99

<131

<52

<29

<26

Leucine

Leu

<286

<215

<87

<70

<64

Phenylalanine

Phe

<342

<306

<178

<123

<98

Tryptophan

Trp

<241

<329

<222

<218

<140

Allo-isoleucine

AlloIle

<3

<6

<1

<2

<4

 

All results reported as nmol/mg creatinine.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Testing Algorithm

Testing includes quantitation of the following amino acids: taurine, threonine, serine, asparagine, hydroxyproline, glutamic acid, glutamine, aspartic acid, ethanolamine, sarcosine, proline, glycine, alanine, citrulline, alpha-aminoadipic acid, alpha-amino-n-butyric acid, valine, cystine, cystathionine, methionine, isoleucine, leucine, tyrosine, phenylalanine, beta-alanine, beta-aminoisobutyric acid, ornithine, lysine, 1-methylhistidine, histidine, 3-methylhistidine, argininosuccinic acid, allo-isoleucine, homocitrulline, gamma-amino-n-butyric acid, hydroxylysine, tryptophan, and arginine.

Sanford Laboratories - Sioux Falls/Rapid City Additional Information:

SANFORD LABORATORY INTERFACE BUILD INFORMATION

Result Code  Result Code Description 
13171 Taurine Urine
13172 Asparagine Urine
13173 Serine Urine
16031 Hydroxyproline Urine
13174 Glycine Urine
13175 Glutamine Urine
16032 Aspartic Acid Urine
16033 Ethanolamine Urine
13176 Histidine Urine
13177 Threonine Urine
13178 Citrulline Urine
16034 Sarcosine Urine
13179 B-Alanine Urine
13180 Alanine Urine
13181 Glutamic Acid Urine
13182 1-Methylhistidine Urine
13183 3-Methylhistidine Urine
16035 Argininosuccinic Acid Urine 
16037 Homocitrulline Urine
13185 Arginine Urine
13186 A-Aminoadipic Acid Urine 
16038 Gamma-Amino-N-Butyric Acid Urine 
13187 B-Aminoisobutyric Acid Urine
13188 A-Amino-N-Butyric Acid Urine
16039 Hydroxylysine Urine
13189 Proline Urine
13190 Ornithine Urine
13191 Cystathionine Urine
13192 Cystine Urine
13193 Lysine Urine
13194 Methionine Urine
13195 Valine Urine
13196 Tyrosine Urine
13197 Isoleucine Urine
13198 Leucine Urine
13199 Phenylalanine Urine
16040 Tryptophan Urine
16041 Allo-Isoleucine Urine
13201 Amino Acid Interpretation 
22900 Reviewed By