Test ID MICR0019 Culture, Acid Fast Bacillus with Stain
AKA
AFB, Acid Fast Bacillus Culture, TB Culture, Mycobacteria
Specimen Type/Requirements
Gastric Contents: Must be processed immediatley after collection. Send to lab promptly.
Respiratory: Bronchial Washings, BAL. Send 10 mL in sterile container.
Sputum: Submit specimen in sterile container. Three-separate collections each with their own order, one of which is a first morning collection, collected at 8-24-hour intervals, is the general recommendation for culturing patients with clinical findings compatible with tuberculosis. 24-hour collections are not acceptable.
Tissue: Collect material using sterile technique. Place in a sterile container with a small amount of sterile saline or on a sterile gauze pad with small amount of sterile saline.
Sterile Fluid: Pleural, throacentesis, etc.: Send 10 mL of fluid in sterile container. CSF: Send 1 mL minimum.
Urine: Submit specimen in sterile container. Three-separate collections each with their own order, one of which is a first morning collection, collected at 8-24-hour intervals, is the general recommendation for culturing patients with clinical findings compatible with tuberculosis. 24-hour collections are not acceptable.
For Blood, please refer to BLOD1118 Culture, Acid Fast Bacillus, Blood for specimen requirements.
Swabs are not acceptable. Anaerobic gel media is not acceptable.
Bone Marrow: Send in a Yellow top SPS Tube or Sterile Container
Stability/Transport
Room Temperature | 7 days | |
---|---|---|
Refrigerated | 7 days (Except CSF, Bone Marrow - See note below) | Preferred for transport |
Frozen | N/A |
Note: CSF and Bone Marrow should be Room Temperature Only
Performed Test Frequency
Monday through Friday
Analytical Time
Stain: Monday through Friday
Culture: 8 -12 weeks
Additional Information
Body fluids: Concentration and Acid Fast Bacilli Stain by Fluorochrome will be performed when there is sufficient sample quantity available at an additional charge.
Identification will be performed if indicated and an additional fee (per organism) may be applied.
Susceptibility testing will only be performed upon request at an additional fee (per organism).
Methodology
Culture/Stain
Performing Lab
Sanford Laboratories Sioux Falls
CPT
87116, 87206, 87015