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HelpTest ID BLOD1108 Estradiol, Serum
Useful For
All applications that require moderately sensitive measurement of estradiol:
-Evaluation of hypogonadism and oligo-amenorrhea in females
-Assessing ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)
-In conjunction with luteinizing hormone measurements, monitoring of estrogen replacement therapy in hypogonadal premenopausal women
-Evaluation of feminization, including gynecomastia, in males
-Diagnosis of estrogen-producing neoplasms in males and, to a lesser degree, females
-As part of the diagnosis and workup of precocious and delayed puberty in females, and, to a lesser degree, males
-As part of the diagnosis and workup of suspected disorders of sex steroid metabolism (eg, aromatase deficiency and 17 alpha-hydroxylase deficiency)
-As an adjunct to clinical assessment, imaging studies and bone mineral density measurement in the fracture risk assessment of postmenopausal women, and, to a lesser degree, older men
-Monitoring low-dose female hormone replacement therapy in postmenopausal women
-Monitoring antiestrogen therapy (eg, aromatase inhibitor therapy)
Specimen Type
Serum RedSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (SST/serum gel tubes are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.2 mL
Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Additional Information: For more information see Steroid Pathways.
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Specimen Minimum Volume
0.7 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Ambient | 28 days | |
| Frozen | 28 days |
Day(s) Performed
Monday through Sunday
Report Available
2 to 6 daysMethod Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82670
Reporting Name
Estradiol, Mass Spectrometry, SReference Values
CHILDREN*
1 to14 days: Estradiol levels in newborns are very elevated at birth but will fall to prepubertal levels within a few days.
Males
|
Tanner stages# |
Mean age |
Reference range |
|
Stage I (>14 days and prepubertal) |
7.1 years |
Undetectable-13 pg/mL |
|
Stage II |
12.1 years |
Undetectable-16 pg/mL |
|
Stage III |
13.6 years |
Undetectable-26 pg/mL |
|
Stage IV |
15.1 years |
Undetectable-38 pg/mL |
|
Stage V |
18 years |
10-40 pg/mL |
#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (± 2) years. For boys, there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
Females
|
Tanner stages# |
Mean age |
Reference range |
|
Stage I (>14 days and prepubertal) |
7.1 years |
Undetectable-20 pg/mL |
|
Stage II |
10.5 years |
Undetectable-24 pg/mL |
|
Stage III |
11.6 years |
Undetectable-60 pg/mL |
|
Stage IV |
12.3 years |
15-85 pg/mL |
|
Stage V |
14.5 years |
15-350 pg/mL** |
#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (± 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
*The reference ranges for children are based on the published literature(1,2), cross-correlation of our assay with assays used to generate the literature data, and on our data for young adults.
ADULTS
Males: 10-40 pg/mL
Females
Premenopausal: 15-350 pg/mL**
Postmenopausal: <10 pg/mL
**E2 levels vary widely through the menstrual cycle.
Conversion factor
E2: pg/mL x 3.676=pmol/L (molecular weight=272)
For International System of Units (SI) conversion of Reference Values, see https://www.mayocliniclabs.com/order-tests/si-unit-conversion.html
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.Special Instructions
Testing Algorithm
For more information see Steroid Pathways.
SANFORD LABORATORY INTERFACE BUILD INFORMATION
| Result Code | Result Code Description |
|---|---|
| 18227 | Estradiol |