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Test ID BLOD0796 Amino Acids, Quantitative, Plasma

Useful For

Evaluating patients with possible inborn errors of metabolism using plasma specimens

 

May aid in evaluation of endocrine disorders, liver diseases, muscle diseases, neoplastic diseases, neurological disorders, nutritional disturbances, kidney failure, and burns

 

Monitoring of patients treated for various inborn errors of metabolism or other causes of amino acid imbalances

Specimen Type

Plasma


Ordering Guidance


Body fluids are not acceptable specimens for this test.

For testing urine specimens, order AAPD / Amino Acids, Quantitative, Random, Urine.

For testing spinal fluid specimens, order AACSF / Amino Acids, Quantitative, Spinal Fluid.



Additional Testing Requirements


Not all patients with homocystinuria/homocystinemia will be detected by this assay. For quantitation of total homocysteine, order CMMPP / Cobalamin, Methionine, and Methylmalonic Acid Pathways, Plasma or HCYSP / Homocysteine, Total, Plasma in conjunction with this amino acids profile.



Shipping Instructions


 



Necessary Information


1. Patient's age is required.

2. Include family history, clinical condition (asymptomatic or acute episode), diet, and drug therapy information.



Specimen Required


Patient Preparation: Patient should fast overnight (4 hours minimum); infants should have specimen collected before next feeding (2-3 hours without total parenteral nutrition if possible).

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Green top (sodium heparin)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Collect specimen and place on wet ice. Note: Thrombin-activated tubes should not be used for collection.

2. Centrifuge immediately or within 4 hours of collection if the specimen is kept at refrigerated temperature.

3. Being careful to ensure that no buffy coat is transferred, aliquot plasma into a plastic vial and freeze.


Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Frozen 14 days

Day(s) Performed

Monday through Friday

Report Available

3 to 5 days

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Portions of this test are covered by patents held by Quest Diagnostics

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82139

Reporting Name

Amino Acids, QN, P

Reference Values

Amino acids

Age groups

<24 months

2-17 years

≥18 years

Taurine (Tau)

31-354

32-181

21-123

Asparagine (Asn)

18-94

25-80

23-94

Serine (Ser)

59-224

53-166

55-146

Hydroxyproline (Hyp)

<121

<73

<38

Glycine (Gly)

80-500

80-500

80-500

Glutamine (Gln)

356-857

353-790

447-774

Aspartic Acid (Asp)

<48

<17

<13

Ethanolamine (EtN)

<70

<30

<20

Histidine (His)

46-147

56-119

61-120

Threonine (Thr)

49-358

48-205

73-325

Citrulline (Cit)

8-42

12-44

18-57

Sarcosine (Sar)

<20

<20

<20

b-Alanine (bAla)

<36

<36

<36

Alanine (Ala)

139-474

144-557

200-579

Glutamic Acid (Glu)

28-376

16-182

13-148

1-Methylhistidine (1MHis)

<12

<12

<12

3-Methylhistidine (3MHis)

<11

<30

<35

Argininosuccinic Acid (Asa)

<5

<5

<5

Homocitruline (Hcit)

<5

<2

<2

Arginine (Arg)

28-164

28-156

45-144

a-Aminoadipic Acid (Aad)

<4

<4

<4

g-Amino-n-butyric Acid (GABA)

<4

<4

<4

b-Aminoisobutyric Acid (bAib)

<9

<5

<5

a-Amino-n-butyric Acid (Abu)

<40

<40

<40

Hydroxylysine (Hyl)

<4

<4

<4

Proline (Pro)

102-342

99-389

107-383

Ornithine (Orn)

32-171

32-148

39-154

Cystathionine (Cth)

<4

<4

<4

Cystine (Cys)

6-131

3-151

8-310

Lysine (Lys)

83-304

61-291

105-335

Methionine (Met)

12-57

13-41

13-40

Valine (Val)

94-382

111-367

134-357

Tyrosine (Tyr)

27-188

36-133

36-113

Isoleucine (IIe)

23-149

26-150

29-153

Leucine (Leu)

59-213

51-216

79-217

Phenylalanine (Phe)

36-105

38-116

45-106

Tryptophan (Trp)

12-103

21-114

21-108

Alloisoleucine (Allolle)

<5

<5

<5

 

All results reported in nmol/mL

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Testing Algorithm

Testing includes quantitation of the following amino acids: taurine, threonine, serine, asparagine, glutamic acid, glutamine, proline, alanine, citrulline, alpha-amino-n-butyric acid, valine, cystine, methionine, isoleucine, leucine, tyrosine, phenylalanine, beta-alanine, ornithine, lysine, histidine, argininosuccinic acid, allo-isoleucine, arginine, hydroxyproline, glycine, aspartic acid, ethanolamine, sarcosine, 1-methylhistidine, 3-methylhistidine, homocitrulline, alpha-aminoadipic acid, gamma-amino-n-butyric acid, beta-aminoisobutyric acid, hydroxylysine, cystathionine, and tryptophan.

 

For more information see Epilepsy: Unexplained Refractory and/or Familial Testing Algorithm.

Sanford Laboratories - Sioux Falls/Rapid City Additional Information:

SANFORD LABORATORY INTERFACE BUILD INFORMATION

Result Code  Result Code Description 
 27932  Interpretation (AAQP)
   
13145 Taurine
13146 Asparagine
13147 Serine
16017 Hydroxyproline
13148 Glycine
13149 Glutamine
16003 Aspartic Acid
16004 Ethanolamine
13150 Histidine
13151 Threonine
13152 Citrulline
16005 Sarcosine
13153 B-Alanine
13154 Alanine
13155 Glutamic Acid
16006 1-Methylhistidine
16007 3-Methylhistidine
13156 Argininosuccinic Acid
16008 Carnosine
16009 Anserine
16010 Homocitrulline
13157 Arginine
16011 Alpha-aminoadipic Acid
16012 Gamma-Amino-N-Butyric Acid
16013 Beta-Aminoisobutyric Acid
13158 A-Amino-N-Butyric Acid
16014 Hydroxylysine
13159 Proline
13160 Ornithine
16015 Cystathionine
13161 Cystine
13162 Lysine
13163 Methionine
13164 Valine
13165 Tyrosine
13166 Isoleucine
13167 Leucine
13168 Phenylalanine
16016 Tryptophan
13170 Allo-isoleucine